Placental growth factor regulates the pentose phosphate pathway and antioxidant defense systems in human retinal endothelial cells.

May 25, 2021 Leave a comment

The molecular mechanisms whereby placental progress issue (PlGF) mediates its results in nonproliferative diabetic retinopathy (DR) are unknown. To raised perceive the position of PlGF in DR, we used tandem mass tags (TMT)-labeled quantitative proteomics to human retinal endothelial cells (HRECs), handled anti-PlGF antibody, and PBS as a management. Practical annotation and pathway enrichments have been carried out, which instructed that the differentially expressed proteins (DEPs) have been concerned in key metabolic processes, protein binding, and membrane, pentose phosphate pathway PPP and adherens junction. We performed built-in gene profiles of our beforehand printed transcriptomic knowledge to the TMT-labeled proteomics knowledge.

The outcomes confirmed the sixty proteins have been discovered to be modified on the mRNA ranges. The purposeful annotation performed for the sixty proteins instructed that 58.3% of proteins have been concerned in PPP, 25% of proteins have been in interleukin-12 singling and 16.7% of proteins have been concerned in glycolysis and gluconeogenesis pathway. Mass spec outcomes have been validated by transendothelial electrical resistance measurement by {an electrical} cell-impedance sensing and western blot evaluation of VE-cadherin, G6PD. These findings counsel that the PPP proteins and antioxidants might act as a downstream goal of PlGF and should play a decisive position in HREC organic features in DR.

PlGF (Placental progress issue) is understood to play a pivotal position in pathological angiogenesis and irritation by stimulating endothelial cell migration and by recruiting pericytes and inflammatory cells comparable to microglia and macrophages. Regardless of the well-defined pathophysiological roles of PlGF, the underlying molecular and mobile mechanisms usually are not utterly understood, particularly the precise relationships between biochemical occasions and molecular pathways regulated by PlGF, whose inhibition displays a protecting position in diabetic retinopathy.

This examine supplies new insights into protein expression patterns and permits the identification of many enticing candidates for investigation of PPP pathway position within the activation of the antioxidant protection system in diabetic retinopathy (DR). Our findings counsel that the PPP proteins and antioxidants (PRDX6, HMOX1, NQO1 and YES1) might act as downstream targets of PlGF and should play a decisive position in HREC organic features in DR.

Era of Uncooked RPPA Information and Their Conversion to Evaluation-Prepared Information.

Reverse section protein array (RPPA) supplies investigators with a strong high-throughput, quantitative, cost-effective know-how for purposeful proteomics research. It’s an antibody-based method with procedures much like that of Western blots. RPPA has all kinds of functions that vary from pharmacodynamics and drug sensitivity evaluation to biomarker discovery, subtype classification, and prediction of affected person prognosis and response to focused remedy. On this paper, we describe the know-how, its limitations, and a few options to beat them. We focus on the steps needed to acquire uncooked RPPA knowledge and convert them into strong, high-quality, analysis-ready knowledge.

We then illustrate the utility of the platform by highlighting some biomarkers and drug responses of most cancers cell strains that affirm earlier findings, as a method to validate the platform and the strategies offered right here. β-Amyloid protein (Aβ) putatively performs a seminal position in synaptic loss in Alzheimer’s illness (AD). Whereas there is no such thing as a consensus relating to the synaptic-relevant species of Aβ, it’s identified that Aβ oligomers (AβOs) are noticeably elevated within the early levels of AD, localizing at or throughout the synapse.

In cell and animal fashions, AβOs have been proven to connect to synapses and instigate synapse dysfunction and deterioration. To determine the pathological mechanism of synaptic loss in AD, will probably be necessary to determine the synaptic targets to which AβOs connect. Herein, we describe all of the steps required for the enrichment, isolation, and identification of SUMOylated substrates from human hepatoma cells and hepatic tissues from a liver most cancers mouse mannequin by utilizing SUBEs.

Placental growth factor regulates the pentose phosphate pathway and antioxidant defense systems in human retinal endothelial cells.

SUMO-Binding Entities (SUBEs) as Instruments for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Most cancers.

Submit-translational modification is a key mechanism regulating protein homeostasis and performance in eukaryotic cells. Amongst all ubiquitin-like proteins in liver most cancers, the modification by SUMO (Small Ubiquitin MOdifier) has been given probably the most consideration. Isolation of endogenous SUMOylated proteins in vivo is difficult as a result of presence of lively SUMO-specific proteases. Preliminary research of SUMOylation in vivo have been based mostly on the molecular detection of particular SUMOylated proteins (e.g., by western blot).

Nonetheless, in lots of instances, antibodies, usually made with non-modified recombinant protein, didn’t immunoprecipitate SUMOylated types of the protein of curiosity. Nickel chromatography has been the opposite strategy to check SUMOylation by capturing histidine-tagged variations of SUMO molecules. This strategy is especially utilized in cells stably expressing or transiently transfected with His-SUMO molecules. To beat these limitations, SUMO-binding entities (SUBEs) have been developed to isolate endogenous SUMOylated proteins.

Starch (Smithies), for electrophoresis
GE6857-500G 500 g
EUR 181

Agarose for DNA electrophoresis
16-0027-500 500 g
EUR 342

Electrophoresis System Set
E1101-E 1 pcs Ask for price

electrophoresis power supply
EV245 ea
EUR 479

CometAssay Electrophoresis System
4250-050-ES 1 Kit
EUR 2950.8

ES 6 Tooth Gel Comb For ES Electrophoresis
LE1011-A4 Ea
EUR 124

myGel Mini Electrophoresis System
BM0286 System
EUR 618

CometAssay Electrophoresis Starter Kit
4250-050-ESK 1 Kit
EUR 3124.4

ES Horizontal Electrophoresis Set
LE1011 Set
EUR 482

6X6 well Electrophoresis Apparatus
EDK626 1 UNIT
EUR 625.5
  • Product category: Benchtop Equipment/Gel Electrophoresis/6x6-well Systems

Glycine
GM2385-100G 100 g
EUR 37

Glycine
GM2385-1KG 1 kg
EUR 50

Glycine
GM2385-250G 250 g
EUR 39

Glycine
GM2385-500G 500 g
EUR 43

Glycine
GM2385-5KG 5 kg
EUR 94

Glycine
HY-Y0966 1g
EUR 119

Glycine
abx082341-500g 500 g
EUR 203
  • Shipped within 5-10 working days.

Glycine
abx082391-500g 500 g
EUR 189
  • Shipped within 5-10 working days.

Glycine
20-abx082501
  • EUR 258.00
  • EUR 189.00
  • EUR 328.00
  • 1 kg
  • 500 g
  • 5 kg
  • Shipped within 5-10 working days.

Glycine
20-abx184699
  • EUR 217.00
  • EUR 370.00
  • 100 g
  • 500 g
  • Shipped within 1-2 weeks.

Glycine
GB0235 500g
EUR 69.14
  • Product category: Electrophoresis Related/Reagents

ES 8/10 Double Gel Comb For ES Electrophoresis
LE1011-A3 Ea
EUR 124

ELISA kit for General Gly (Glycine)
ELK8276 1 plate of 96 wells
EUR 372
  • A monoclonal antibody specific to Glycine (Gly) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glycine (Gly) and unlabeled Glycine (Gly) (Standards or samples) with the pre-coated antibody
  • Show more
Description: A competitive Inhibition ELISA kit for detection of Glycine from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

electrophoresis ps 400v, 500ma, 50w
EV1450 ea
EUR 479

electrophoresis ps 400v, 500ma, 50w
EV1450-UK ea
EUR 479

electrophoresis ps 400v, 500ma, 50w
EV1450-US ea
EUR 479

electrophoresis ps 1500v, 300ma, 150w
EV2230 ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w
EV2230-UK ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w
EV2230-US ea
EUR 749

electrophoresis ps 300v, 1000ma, 150w
EV2310 ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w
EV2310-UK ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w
EV2310-US ea
EUR 572

electrophoresis ps 3000v, 150ma, 150w
EV2320 ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w
EV2320-UK ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w
EV2320-US ea
EUR 749

electrophoresis power supply uk plug
EV245-UK ea
EUR 479

electrophoresis power supply 120 vac
EV245-US ea
EUR 479

electrophoresis ps 600v, 500ma, 150w
EV2650 ea
EUR 572

electrophoresis ps 600v, 500ma, 150w
EV2650-UK ea
EUR 572

electrophoresis ps 600v, 500ma, 150w
EV2650-US ea
EUR 572

electrophoresis ps 300v, 2000ma, 300w
EV3020 ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w
EV3020-UK ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w
EV3020-US ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150 ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150-UK ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w
EV3150-US ea
EUR 800

electrophoresis ps 3000v, 300ma, 300w
EV3330 ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w
EV3330-UK ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w
EV3330-US ea
EUR 1214

electrophoresis ps 600v, 1000ma, 300w
EV3610 ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w
EV3610-UK ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w
EV3610-US ea
EUR 800

electrophoresis ps 6000v, 150ma, 300w
EV3620 ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w
EV3620-UK ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w
EV3620-US ea
EUR 1214

ELISA kit for Human GLDC (Glycine Dehydrogenase)
E-EL-H5572 1 plate of 96 wells
EUR 534
  • Gentaur's GLDC ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLDC. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human GLDC (Glycine Dehydrogenase) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Glycine N-methyltransferase
EK3139 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Glycine N-methyltransferase in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Human GLDC (Glycine Dehydrogenase)
ELK4740 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyci
  • Show more
Description: A sandwich ELISA kit for detection of Glycine Dehydrogenase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse GLDC (Glycine Dehydrogenase)
ELK6991 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyci
  • Show more
Description: A sandwich ELISA kit for detection of Glycine Dehydrogenase from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Rat GLDC (Glycine Dehydrogenase)
ELK7167 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyci
  • Show more
Description: A sandwich ELISA kit for detection of Glycine Dehydrogenase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Glycine, 99%
G0610-100 1Kg
EUR 134

Glycine, 99%
G0610-500 5kg
EUR 301

Glycine hydrochloride
GE1802-100G 100 g
EUR 61

Glycine hydrochloride
GE1802-1KG 1 kg
EUR 229

Glycine hydrochloride
GE1802-250G 250 g
EUR 94

Glycine hydrochloride
GE1802-25G 25 g
EUR 45

Glycine hydrochloride
GE1802-500G 500 g
EUR 142

Glycine (BSA)
20-abx165740
  • EUR 606.00
  • EUR 258.00
  • EUR 1817.00
  • EUR 718.00
  • EUR 439.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Glycine (OVA)
20-abx165741
  • EUR 620.00
  • EUR 272.00
  • EUR 1859.00
  • EUR 732.00
  • EUR 453.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

IOX2(Glycine)
A4189-10 10 mg
EUR 154
Description: IOX2 is a potent and selective inhibitor of HIF-1? prolyl hydroxylase-2 (PHD2) with an IC50 of 21 nM for PHD2/ELGN-1 in a cell-free assay, IOX2 has shown >100-fold selectivity over JMJD2A, JMJD2C, JMJD2E, JMJD3, or the 2OG oxygenase FIH [1].

IOX2(Glycine)
A4189-5.1 10 mM (in 1mL DMSO)
EUR 148
Description: IOX2 is a potent and selective inhibitor of HIF-1? prolyl hydroxylase-2 (PHD2) with an IC50 of 21 nM for PHD2/ELGN-1 in a cell-free assay, IOX2 has shown >100-fold selectivity over JMJD2A, JMJD2C, JMJD2E, JMJD3, or the 2OG oxygenase FIH [1].

IOX2(Glycine)
A4189-50 50 mg
EUR 456
Description: IOX2 is a potent and selective inhibitor of HIF-1? prolyl hydroxylase-2 (PHD2) with an IC50 of 21 nM for PHD2/ELGN-1 in a cell-free assay, IOX2 has shown >100-fold selectivity over JMJD2A, JMJD2C, JMJD2E, JMJD3, or the 2OG oxygenase FIH [1].

IOX2(Glycine)
A4189-S Evaluation Sample
EUR 81
Description: IOX2 is a potent and selective inhibitor of HIF-1? prolyl hydroxylase-2 (PHD2) with an IC50 of 21 nM for PHD2/ELGN-1 in a cell-free assay, IOX2 has shown >100-fold selectivity over JMJD2A, JMJD2C, JMJD2E, JMJD3, or the 2OG oxygenase FIH [1].

Glycine [BSA]
DAG3313 250ug
EUR 715

250G Glycine
NAT1046 250G
EUR 79

Glycine Receptor
RA15025 200 ug
EUR 552

CometAssay Single Cell Gel Electrophoresis Assay
4250-050-K 50 tests
EUR 428

Electrophoresis Apparatus - 7x10cm (6-10-well)
EDK611 1 UNIT
EUR 319.26
  • Product category: Benchtop Equipment/Gel Electrophoresis/6-10-well Systems

Electrophoresis Apparatus - 7x14cm (6-10-well)
EDK613 1 UNIT
EUR 406.26
  • Product category: Benchtop Equipment/Gel Electrophoresis/6-10-well Systems

10-well protein Vertical Electrophoresis Apparatus
EDK692 1 UNIT
EUR 406.26
  • Product category: Benchtop Equipment/Gel Electrophoresis/10-well Systems

ELISA kit for Human GLYT1 (Glycine Transporter 1)
ELK4106 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Transporter 1 (GLYT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyc
  • Show more
Description: A sandwich ELISA kit for detection of Glycine Transporter 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human GNMT (Glycine-N-Methyltransferase)
ELK4155 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine-N-Methyltransferase (GNMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to
  • Show more
Description: A sandwich ELISA kit for detection of Glycine-N-Methyltransferase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Rat GNMT (Glycine N-methyltransferase)
E-EL-R1105 1 plate of 96 wells
EUR 534
  • Gentaur's GNMT ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GNMT. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat GNMT (Glycine N-methyltransferase) in samples from Serum, Plasma, Cell supernatant

L-Homopropargyl Glycine
C3406-10 10 mg
EUR 112
Description: L-homopropargylglycine (Hpg), an alkyne-tagged analog of methionine, has been used to selectively tag newly synthesized mammalian proteins in a method similar to conventional pulse-chase labeling, and labeled proteins were visualized via fluorescence.

L-Homopropargyl Glycine
C3406-100 100 mg
EUR 467
Description: L-homopropargylglycine (Hpg), an alkyne-tagged analog of methionine, has been used to selectively tag newly synthesized mammalian proteins in a method similar to conventional pulse-chase labeling, and labeled proteins were visualized via fluorescence.

L-Homopropargyl Glycine
C3406-50 50 mg
EUR 293
Description: L-homopropargylglycine (Hpg), an alkyne-tagged analog of methionine, has been used to selectively tag newly synthesized mammalian proteins in a method similar to conventional pulse-chase labeling, and labeled proteins were visualized via fluorescence.

N-Oleoyl glycine
HY-113204 10mg
EUR 142

N-(Phosphonomethyl)glycine
GM3149-100G 100 g
EUR 198

N-(Phosphonomethyl)glycine
GM3149-25G 25 g
EUR 94

N-(Phosphonomethyl)glycine
GM3149-5G 5 g
EUR 62

Glycine Amidinotransferase (Recombinant)
20-abx073791
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Glycine (Gly) Antibody
20-abx130518
  • EUR 439.00
  • EUR 133.00
  • EUR 1274.00
  • EUR 606.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Cbz-Glycine hydrazide
20-abx183928
  • EUR 453.00
  • EUR 1163.00
  • 10 g
  • 50 g
  • Shipped within 1-2 weeks.

N-Benzyl glycine
20-abx184261
  • EUR 356.00
  • EUR 230.00
  • 100 g
  • 25 g
  • Shipped within 1-2 weeks.

Glycine Oxidase Antibody
A1116-100
EUR 338

Glycine Oxidase Antibody
A1116-30T
EUR 146

Glycine dehydrogenase antibody
23002-100ul 100ul
EUR 390

Glycine dehydrogenase antibody
70R-13355 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal Glycine dehydrogenase antibody

1KG Glycine Ultrapure
NAT1048 1KG
EUR 107

Glycine Assay Kit
MET-5070 100 assays
EUR 450

ELISA kit for Rat GLRa2 (Glycine Receptor Alpha 2)
ELK7267 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Receptor Alpha 2 (GLR?2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G
  • Show more
Description: A sandwich ELISA kit for detection of Glycine Receptor Alpha 2 from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

5-ROX glycine *Fluorescence reference standard for PCR reactions*
396 25 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

myGel Mini Electrophoresis System 230V (includes transformer)
BM0291 System
EUR 658

CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K 1 kit
EUR 570.8

ES Electrophoresis Casting Tray, 7cm X 14cm
LE1011-A1 Ea
EUR 165

ES Electrophoresis Casting Tray, 7cm X 7cm
LE1011-A2 Ea
EUR 151

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange 1 prep
EUR 158

ELISA kit for Rat Glycine receptor subunit alpha-2 (GLRA2)
KTE100839-48T 48T
EUR 332
  • The GLRA2 gene encodes the alpha-2 subunit of the glycine receptor. The alpha-1 subunit is encoded by the GLRA1 gene, which maps to chromosome 5q32. The GLRA2 gene is expressed in fetal brain and spinal cord, and the GLRA1 gene is expressed predomina
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Glycine receptor subunit alpha-2 (GLRA2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Glycine receptor subunit alpha-2 (GLRA2)
KTE100839-5platesof96wells 5 plates of 96 wells
EUR 2115
  • The GLRA2 gene encodes the alpha-2 subunit of the glycine receptor. The alpha-1 subunit is encoded by the GLRA1 gene, which maps to chromosome 5q32. The GLRA2 gene is expressed in fetal brain and spinal cord, and the GLRA1 gene is expressed predomina
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Glycine receptor subunit alpha-2 (GLRA2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Glycine receptor subunit alpha-2 (GLRA2)
KTE100839-96T 96T
EUR 539
  • The GLRA2 gene encodes the alpha-2 subunit of the glycine receptor. The alpha-1 subunit is encoded by the GLRA1 gene, which maps to chromosome 5q32. The GLRA2 gene is expressed in fetal brain and spinal cord, and the GLRA1 gene is expressed predomina
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Glycine receptor subunit alpha-2 (GLRA2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Peptidyl-glycine alpha-amidating monooxygenase (PAM)
KTE61310-48T 48T
EUR 332
  • Peptidyl-glycine alpha-amidating monooxygenase is a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Peptidyl-glycine alpha-amidating monooxygenase (PAM)
KTE61310-5platesof96wells 5 plates of 96 wells
EUR 2115
  • Peptidyl-glycine alpha-amidating monooxygenase is a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Peptidyl-glycine alpha-amidating monooxygenase (PAM)
KTE61310-96T 96T
EUR 539
  • Peptidyl-glycine alpha-amidating monooxygenase is a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Peptidyl-glycine alpha-amidating monooxygenase (PAM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Glycine (Molecular Biology Grade)
CE158 1 kg
EUR 70

Glycine (Molecular Biology Grade)
CE159 5 kg
EUR 190

N-(2-Aminoethyl)glycine
GM1937-1G 1 g
EUR 269

Glycine, EP, USP grade
GM2320-100G 100 g
EUR 40

Glycine, EP, USP grade
GM2320-1KG 1 kg
EUR 62

Glycine, EP, USP grade
GM2320-250G 250 g
EUR 45

Glycine, EP, USP grade
GM2320-500G 500 g
EUR 50

Glycine, EP, USP grade
GM2320-5KG 5 kg
EUR 142

Glycine ethyl ester hydrochloride
GM6023-100G 100 g
EUR 44

Glycine ethyl ester hydrochloride
GM6023-1KG 1 kg
EUR 107

Glycine ethyl ester hydrochloride
GM6023-2500G 2500 g
EUR 205

Glycine ethyl ester hydrochloride
GM6023-250G 250 g
EUR 56

Glycine ethyl ester hydrochloride
GM6023-500G 500 g
EUR 74

Glycine benzyl ester hydrochloride
GM7497-100G 100 g
EUR 150

Glycine N-Methyltransferase (Recombinant)
20-abx073053
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.

Glycine C-Acetyltransferase (Recombinant)
20-abx073394
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Glycine-N-Acyltransferase (Recombinant)
20-abx073713
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
20-abx126917
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
20-abx112798
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glycine Dehydrogenase (GLDC) Antibody
20-abx129785
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Glycine Oxidase (thiO) Antibody
20-abx109408
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glycine oxidase Antibody (HRP)
20-abx109409
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glycine Receptor Polyclonal Antibody
ABP-PAB-22022 100 ug Ask for price
    • Product line: Pan-specific Antibodies
    • Brand:

Glycine Dehydrogenase (GLDC) Antibody
20-abx135853
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glycine Dehydrogenase (GLDC) Antibody
20-abx102152
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Glycine Dehydrogenase (GLDC) Antibody
20-abx102153
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Glycine oxidase Antibody (Biotin)
20-abx106227
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glycine oxidase Antibody (FITC)
20-abx107641
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Glycine Dehydrogenase (GLDC) Antibody
abx034398-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Glycine Dehydrogenase (GLDC) Antibody
abx034398-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
abx025718-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
abx025718-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
20-abx005060
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
abx031173-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Glycine Amidinotransferase (GATM) Antibody
abx031173-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Glycine Benzyl Ester Hydrochloride
abx188724-250g 250 g
EUR 565
  • Shipped within 1-2 weeks.

Glycine Dehydrogenase (GLDC) Antibody
20-abx172626
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug
  • Please enquire.

Glycine Dehydrogenase (GLDC) Antibody
20-abx176666
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug
  • Please enquire.

Glycine Dehydrogenase (GLDC) Antibody
20-abx176667
  • EUR 1302.00
  • EUR 620.00
  • 1 mg
  • 200 ug
  • Please enquire.

Glycine Amidinotransferase (GATM) Antibody
20-abx322337
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Glycine (Gly) ELISA Kit
20-abx258882
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Glycine (Gly) CLIA Kit
20-abx490614
  • EUR 8976.00
  • EUR 4779.00
  • EUR 1099.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Glycine Amidinotransferase (GATM) Antibody
abx233368-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Glycine Dehydrogenase (GLDC) Antibody
abx233476-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

Boc-Glycine methyl ester
20-abx182385
  • EUR 286.00
  • EUR 648.00
  • 100 g
  • 500 g
  • Shipped within 1-2 weeks.

Glycine max lectin (SBA)
05-0117-10 10 mg
EUR 132

Glycine max lectin (SBA)
05-0117-1000 1 g Ask for price

Glycine max lectin (SBA)
05-0117-50 50 mg
EUR 424

Glycine max 2S albumin
1-CSB-YP324829GGV
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 18.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Glycine max 2S albumin expressed in Yeast

Firstly, we describe strategies concerned within the preparation and lysis of the human hepatoma cells and liver tumor tissue samples. Then, an intensive rationalization of the preparation of SUBEs and controls is detailed together with the protocol for the protein pull-down assays. Lastly, some examples are supplied relating to the choices accessible for the identification and characterization of the SUMOylated proteome, particularly the usage of western–blot evaluation for the detection of a particular SUMOylated substrate from liver tumors or the usage of proteomics by mass spectrometry for high-throughput characterization of the SUMOylated proteome and interactome in hepatoma cells.

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