Proteomic Analysis of Differentially Expressed Proteins in the Placenta of Anticardiolipin Antibody- (ACA-) Positive Pregnant Mice after Anzi Heji Treatment

Anzi Heji (AZHJ) has been used to deal with anticardiolipin antibody– (ACA-) optimistic pregnant ladies liable to spontaneous abortion for a few years. The intention of this research was to research the protecting mechanism of AZHJ in a mouse mannequin of ACA-positive being pregnant liable to spontaneous abortion utilizing label-free quantitative proteomics. Mice have been divided into three teams: regular pregnant mice (management group), ACA-positive pregnant mice administered regular saline (mannequin group), and ACA-positive pregnant mice administered AZHJ (AZHJ group).

The mannequin was established by injecting βproteomic and bioinformatic analyses. The degrees of IL-6, IL-10, ACA, and TNF-α within the serum and placentas of the mice have been measured by enzyme-linked immunosorbent assays (ELISAs). Proteomic information have been validated by western blot evaluation. The irregular serum and placental ranges of IL-6, ACA, and TNF-α within the mannequin group have been reversed by AZHJ. There have been 39 upregulated and 10 downregulated DEPs within the AZHJ group relative to the mannequin group.

Bioinformatic evaluation revealed that the DEPs have been primarily concerned in nucleic acid binding, sign conduction, and posttranslational modification. The placental ranges of T-cell immunoglobulin mucin 3 (Tim-3) and Toll-like receptor 4 (TLR4) expression and AKT phosphorylation within the three teams have been per the proteomic findings. Tim-3/AKT signaling is concerned in maternal-fetal immune tolerance, whereas TLR4 is related to inflammatory responses. Collectively, these outcomes point out that AZHJ might exert its protecting impact in ACA-positive pregnant mice by regulating the maternal-fetal immune tolerance and inflammatory response.

Merchandise have been subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) evaluation and searched towards a UniProt Homo sapiens database. Relative protein abundance was decided for every pattern. Primarily based on proteomics outcomes, lumican was measured by enzyme-linked immunosorbent assay (ELISA) and Western blot evaluation was carried out for interleukin-1 receptor antagonist (IL-1Ra) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2). Cell viability was decided by calcein AM (stay) and ethidium homodimer (lifeless) staining and confocal microscopy.

Proteomic evaluation revealed 919 proteins within the 9 merchandise. Proteins have been primarily collagens, keratin, and albumin. Lumican, a small leucine-rich proteoglycan (SLRP) was present in all samples. Western blot evaluation for IL-1Ra and TIMP-2 indicated presence of each proteins, with nonspecific antibody binding additionally current in all samples. No stay cells have been recognized in any product.

Schwann Cell Stimulation of Pancreatic Most cancers Cells: A Proteomic Evaluation

Schwann cells (SCs), the glial element of peripheral nerves, have been recognized as promoters of pancreatic most cancers (PC) development, however the molecular mechanisms are unclear. Within the current research, we aimed to establish proteins launched by SCs that might stimulate PC progress and invasion. Proteomic evaluation of human major SC secretome was carried out utilizing liquid chromatography-tandem mass spectrometry, and a complete of 13,796 distinctive peptides comparable to 1,470 particular person proteins have been recognized.

Metabolic and cell-cell adhesion pathways confirmed the best ranges of enrichment, a discovering in step with the supportive function of SCs in peripheral nerves. We recognized seven SC-secreted proteins that have been validated by western blot. The involvement of those SC-secreted proteins was additional demonstrated by utilizing blocking antibodies. PC cell proliferation and invasion induced by SC-conditioned media have been decreased utilizing blocking antibodies towards the matrix metalloproteinase-2, cathepsin D, plasminogen activator inhibitor-1, and galectin-1.

Blocking antibodies towards the proteoglycan biglycan, galectin-Three binding protein, and tissue inhibitor of metalloproteinases-2 decreased solely the proliferation however not the invasion of PC cells. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment have been performed utilizing the Database for Annotation, Visualization, and Built-in Discovery. The elevated expression of phosphorylated ARPC2 discovered within the PBMCs of our sufferers, and its affiliation with derangement of F-actin filaments, would possibly recommend that proteins regulating actin dynamics in immune cells might be concerned within the mechanism of CAMR of kidney grafts.

Collectively, this research delineates the secretome of human SCs and identifies proteins that may stimulate PC cell progress and invasion and subsequently represent potential therapeutic targets. Altogether, 503 lysine succinylation websites in 303 proteins have been recognized. Lactate dehydrogenase A (LDHA), the important thing enzyme in Warburg impact, was discovered extremely succinylated at Ok222 in GC. Intriguingly, this modification didn’t have an effect on LDHA ubiquitination, however decreased the binding of ubiquitinated LDHA to SQSTM1, thereby lowering its lysosomal degradation.

Proteomic Analysis of Differentially Expressed Proteins in the Placenta of Anticardiolipin Antibody- (ACA-) Positive Pregnant Mice after Anzi Heji Treatment

Altered Phosphorylation of Cytoskeleton Proteins in Peripheral Blood Mononuclear Cells Characterizes Power Antibody-Mediated Rejection in Kidney Transplantation

Power antibody-mediated rejection (CAMR) is the key reason for kidney transplant failure. The molecular mechanisms underlying this occasion are nonetheless poorly outlined and this lack of understanding deeply influences the potential therapeutic methods. The intention of our research was to investigate the phosphoproteome of peripheral blood mononuclear cells (PBMCs), to establish mobile signaling networks differentially activated in CAMR. Phosphoproteins remoted from PBMCs of biopsy confirmed CAMR, kidney transplant recipients with regular graft operate and histology and wholesome immunocompetent people, have been investigated by proteomic evaluation.

HemoVoid™ LC-MS On-Bead For Erythrocytes Proteomics
HVB-MS10 10 preps
EUR 521
Description: Hemoglobin Removal Kit
AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE
AGR-MS-1001 1/pk
EUR 2038
Description: Bioscience Mol Bio; Agarose
LC-LC(+)-Biotin
abx184150-5g 5 g
EUR 996
  • Shipped within 1-2 weeks.
Human Osteonectin (ON) ELISA Kit
DLR-ON-Hu-48T 48T
EUR 479
  • Should the Human Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Osteonectin (ON) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Osteonectin (ON) ELISA Kit
DLR-ON-Hu-96T 96T
EUR 621
  • Should the Human Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Osteonectin (ON) in samples from serum, plasma, tissue homogenates or other biological fluids.
Mouse Osteonectin (ON) ELISA Kit
DLR-ON-Mu-48T 48T
EUR 489
  • Should the Mouse Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Osteonectin (ON) ELISA Kit
DLR-ON-Mu-96T 96T
EUR 635
  • Should the Mouse Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Osteonectin (ON) ELISA Kit
DLR-ON-Ra-48T 48T
EUR 508
  • Should the Rat Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Osteonectin (ON) ELISA Kit
DLR-ON-Ra-96T 96T
EUR 661
  • Should the Rat Osteonectin (ON) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Osteonectin (ON) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Osteonectin (ON) ELISA Kit
RD-ON-Hu-48Tests 48 Tests
EUR 478
Human Osteonectin (ON) ELISA Kit
RD-ON-Hu-96Tests 96 Tests
EUR 662
Mouse Osteonectin (ON) ELISA Kit
RD-ON-Mu-48Tests 48 Tests
EUR 489
Mouse Osteonectin (ON) ELISA Kit
RD-ON-Mu-96Tests 96 Tests
EUR 677
Rat Osteonectin (ON) ELISA Kit
RD-ON-Ra-48Tests 48 Tests
EUR 511
Rat Osteonectin (ON) ELISA Kit
RD-ON-Ra-96Tests 96 Tests
EUR 709
Human Osteonectin (ON) ELISA Kit
RDR-ON-Hu-48Tests 48 Tests
EUR 500
Human Osteonectin (ON) ELISA Kit
RDR-ON-Hu-96Tests 96 Tests
EUR 692
Mouse Osteonectin (ON) ELISA Kit
RDR-ON-Mu-48Tests 48 Tests
EUR 511
Mouse Osteonectin (ON) ELISA Kit
RDR-ON-Mu-96Tests 96 Tests
EUR 709
Rat Osteonectin (ON) ELISA Kit
RDR-ON-Ra-48Tests 48 Tests
EUR 534
Rat Osteonectin (ON) ELISA Kit
RDR-ON-Ra-96Tests 96 Tests
EUR 742
AlbuVoid™ Kit
AVK-05 5 preps
EUR 405
Description: Albumin Removal Kit
AlbuVoid™ Kit
AVK-10 10 preps
EUR 552
Description: Albumin Removal Kit
AlbuVoid™ Kit
AVK-50 50 preps
EUR 1547
Description: Albumin Removal Kit
Trypsin, Recombinant, Proteomics grade
P1228-1000
EUR 196
Trypsin, Recombinant, Proteomics grade
P1228-10000
EUR 1224
Trypsin, Recombinant, Proteomics grade
P1228-5000
EUR 615
Biotin-LC-LC-NHS
2346-1000
EUR 1675
Biotin-LC-LC-NHS
2346-250
EUR 588
Biotin-LC-LC-NHS
2346-50
EUR 196
Biotin-LC-LC-NHS
2346-5000 Ask for price
AlbuVoid Binding Buffer AVBB
AVBB-1000 1000 mL
EUR 456
Description: Albuvoid™ Buffer
AlbuVoid Binding Buffer AVBB
AVBB-500 500 mL
EUR 245
Description: Albuvoid™ Buffer
AlbuVoid Buffer Kit - 1000
AVBK-1000 1000 mL
EUR 1166
Description: Albuvoid™ Buffer
AlbuVoid Buffer Kit - 500
AVBK-500 500 mL
EUR 615
Description: Albuvoid™ Buffer
AlbuVoid Elution Buffer AVEB
AVEB-1000 1000 mL
EUR 456
Description: Albuvoid™ Buffer
AlbuVoid Elution Buffer AVEB
AVEB-500 500 mL
EUR 245
Description: Albuvoid™ Buffer
AlbuVoid Wash Buffer AVWB
AVWB-1000 1000 mL
EUR 456
Description: Albuvoid™ Buffer
AlbuVoid Wash Buffer AVWB
AVWB-500 500 mL
EUR 245
Description: Albuvoid™ Buffer
AlbuVoid™ PLUS Kit
NP-AVK-05 5 preps
EUR 405
Description: Albumin Removal Kit
AlbuVoid™ PLUS Kit
NP-AVK10 10 preps
EUR 587
Description: Albumin Removal Kit
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)
7097-25
EUR 222
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (3 biotin/BSA)
7097-5
EUR 115
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)
7098-25
EUR 233
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)
7098-5
EUR 120
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)
7099-25
EUR 244
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (12 biotin/BSA)
7099-5
EUR 131
101Bio Protein Solubilization Reagent for MS
P5W11 NULL
EUR 0
Micro Bead Sterlizer
B1201-E 1 PC
EUR 542.7
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
Micro Bead Sterlizer
B1202-E 1 PC
EUR 84.14
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
MS-1020
GL5031-1MG 1 mg
EUR 160
MS 275
abx076834-1mg 1 mg
EUR 314
  • Shipped within 5-12 working days.
Ms-275
20-abx182582
  • EUR 509.00
  • EUR 300.00
  • 100 mg
  • 10 mg
  • Shipped within 1-2 weeks.
MS-1020
1911-1
EUR 164
Refill Glass Beads
B1201-BEAD 1 PC
EUR 117.78
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.
LC-Protein Kinase G Substrate; LC-Gsubtide (Biotin)
20-abx265657
  • EUR 467.00
  • EUR 773.00
  • EUR 356.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
ELISA kit for Human MS (Melatonin Sulfate)
E-EL-H1726 1 plate of 96 wells
EUR 534
  • Gentaur's MS ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human MS. During the reaction, Human MS in the sample or standard competes with a fixed amount of Human MS on the sol
  • Show more
Description: A competitive ELISA kit for quantitative measurement of Human MS (Melatonin Sulfate) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Melatonin sulfate (MS)
KTE61523-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Human Melatonin sulfate (MS) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
sulfo-LC-SPDP
ADC-L-133 unit Ask for price
Biotin LC hydrazide
HY-101885 5mg
EUR 119
CD224 LC Antibody
20-abx121054
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Enteropeptidase LC Antibody
20-abx121089
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
MASP1 LC Antibody
20-abx121154
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Myeloperoxidase LC Antibody
20-abx121172
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
CD49e LC Antibody
20-abx009097
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
CD49f LC Antibody
20-abx009102
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
sulfo-LC-(+)-Biotin
20-abx185570
  • EUR 370.00
  • EUR 1261.00
  • EUR 578.00
  • 100 mg
  • 1 g
  • 250 mg
  • Shipped within 1-2 weeks.
LC-(+)-Biotin hydrazide
abx185659-1g 1 g
EUR 648
  • Shipped within 1-2 weeks.
LC-NHS-(+)-Biotin
20-abx185861
  • EUR 634.00
  • EUR 398.00
  • 1 g
  • 250 mg
  • Shipped within 1-2 weeks.
CD49f LC antibody
70R-49925 100 ul
EUR 244
Description: Purified Polyclonal CD49f LC antibody
CD49e LC antibody
70R-49932 100 ul
EUR 244
Description: Purified Polyclonal CD49e LC antibody
NHS-LC-Biotin
A8004-100 100 mg
EUR 119
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end.
NHS-LC-Biotin
A8004-1000 1 g
EUR 548
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end.
Iodoacetyl-LC-Biotin
A8009-100 100 mg
EUR 264
Description: Iodoacetyl-LC-Biotin (N-iodoacetyl-N-biotinylhexylenediamine), a sulfhydryl-reactive biotinylation agent, is a water-insoluble reagent that requires the dissolution of suitable solvents, including dimethyl sulfoxide (DMSO) and dimethylformamide (DMF).
Biotin-LC-Kemptide
5-00800 4 x 1mg Ask for price
Kininogen LC antibody
23031-100ul 100ul
EUR 390
Sulfo-LC-SPDP
2325-250
EUR 1567
Sulfo-LC-SPDP
2325-50
EUR 436
Biotin-LC-NHS
2345-1000
EUR 1001
Biotin-LC-NHS
2345-250
EUR 419
Biotin-LC-NHS
2345-50
EUR 142
pPINK- LC Plasmid
PVT4035 2 ug
EUR 325
BrightGreen miRNA qPCR MasterMix-No Dye
MasterMix-mS 4 x 1.25 ml for 500 reactions
EUR 151
Bangs Lab Bead Solution
SOLN1-1000 1000 ML
EUR 155.06
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
Bangs Lab Bead Solution
SOLN1-2000 2000 ML
EUR 212.7
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
Bangs Lab Bead Solution
SOLN1-500 500 ML
EUR 98.51
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres
uv lamp 2x15w, 254/365nm
VL215-LC ea
EUR 817
uv lamp 2x6w, 254/365nm
VL6-LC ea
EUR 538
darkroom 254/365nm
VLCN15-LC ea
EUR 1535
ELISA kit for Mouse ON (Osteonectin)
E-EL-M0867 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON. Standards or samples are added to the micro ELISA plate wells and combined with the sp
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human ON (Osteonectin)
ELK1682 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Rat ON (Osteonectin)
ELK1683 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
ELISA kit for Mouse ON (Osteonectin)
ELK1684 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteonectin (ON). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteonectin (ON).
  • Show more
Description: A sandwich ELISA kit for detection of Osteonectin from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
CLIA kit for Human ON (Osteonectin)
E-CL-H0889 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ON . Standards or samples are added to the micro CLIA plate wells and combined with the spec
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Human ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
CLIA kit for Mouse ON (Osteonectin)
E-CL-M0530 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ON . Standards or samples are added to the micro CLIA plate wells and combined with the spec
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Mouse ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Rat ON (Osteonectin)
E-EL-R0701 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the micro ELISA plate wells and combined with the spec
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
CLIA kit for Rat ON (Osteonectin)
E-CL-R0498 1 plate of 96 wells
EUR 584
  • Gentaur's ON CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat ON . Standards or samples are added to the micro CLIA plate wells and combined with the specif
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Rat ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human ON (Osteonectin)
E-EL-H1351 1 plate of 96 wells
EUR 534
  • Gentaur's ON ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro ELISA plate wells and combined with the sp
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human ON (Osteonectin) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human Osteonectin (ON)
KTE60336-48T 48T
EUR 354
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-5platesof96wells 5 plates of 96 wells
EUR 2252
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Osteonectin (ON)
KTE60336-96T 96T
EUR 572
  • Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone min
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Osteonectin (ON) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
MS 245 oxalate
B5540-10 10 mg
EUR 292
MS 245 oxalate
B5540-5 5 mg
EUR 210
MS-275 (Entinostat)
E1KS1053 25 mg
EUR 247
Methyl-PEG3-MS
abx186609-250mg 250 mg
EUR 439
  • Shipped within 1-2 weeks.
Biotin-LC-Protein Kinase G Substrate; Biotin-LC-G-Subtide
5-00802 4 x 5mg Ask for price
Polyclonal CD49e LC Antibody
APR05533G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CD49e LC . This antibody is tested and proven to work in the following applications:
Cathepsin C LC Antibody
20-abx121043
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
CD41 LC 1 Antibody
20-abx121061
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Factor IX LC Antibody
20-abx121094
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Factor XI LC Antibody
20-abx121097
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
CD224 LC Blocking Peptide
20-abx161054
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
Enteropeptidase LC Blocking Peptide
20-abx161089
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
MASP1 LC Blocking Peptide
20-abx161154
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
Myeloperoxidase LC Blocking Peptide
20-abx161172
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
CD49f LC Blocking Peptide
20-abx062800
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
CD49e LC Blocking Peptide
20-abx062807
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.
Cathepsin D LC Antibody
20-abx009376
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Complement C1R LC Antibody
20-abx009532
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
LC-Kemptide (Biotin) Peptide
20-abx265999
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.
Complement C1R LC antibody
70R-49435 100 ul
EUR 244
Description: Purified Polyclonal Complement C1R LC antibody
Cathepsin D LC antibody
70R-49618 100 ul
EUR 244
Description: Purified Polyclonal Cathepsin D LC antibody
Sulfo-NHS-LC-Biotin
A8003-100 100 mg
EUR 142
Description: Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent.
Sulfo-NHS-LC-Biotin
A8003-1000 1 g
EUR 572
Description: Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent.
Sulfo-NHS-LC-Biotin
2326-100
EUR 311
Sulfo-NHS-LC-Biotin
2326-50
EUR 202
Sulfo-NHS-LC-Biotin
2326-set
EUR 213
ExoFACS? Kit for Serum Exosomes
K1233-20
EUR 794
AffiPure Ni-NTA Agarose Bead,For His-Tagged Protein Purification
N3505-010 10ml
EUR 317
AffiPure Ni-NTA Agarose Bead,For His-Tagged Protein Purification
N3505-050 50ml
EUR 833
ON/ Rat ON ELISA Kit
ELA-E0791r 96 Tests
EUR 886
Bead Sample Pack - 50mL Set
BSP-50B2 1pack
EUR 404
Description: Bead sample pack for Bullet Blender 50mL tube models. Includes 20mL of: ZROB05, ZROB20, SSB02, SSB14B, SSB48, SSB60, SSUFO35 and SSUFO56.
Bead Sample Pack - 5E Set
BSP-5E 1pack
EUR 252
Description: Bead sample pack for Bullet Blender 5E models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB48, SSUFO35 and SSUFO56.
Bead Sample Pack - 5M Set
BSP-5Y 1pack
EUR 241
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35.
Bead Sample Pack - Full Set
BSP-ALL2 1pack
EUR 384
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes.
Bead Sample Pack - Cell Cultures
BSP-CC2 1pack
EUR 195
Description: Bead sample pack for homogenization of cell cultures. Includes 10mL of: GB01, GB05, ZROB015, ZROB05 and SSB02.
Bead Sample Pack - Microcentrifuge Set
BSP-MC2 1pack
EUR 232
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35.
Bead Sample Pack - Organ Tissues
BSP-OT3 1pack
EUR 235
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35.
DEAE Rapid Run Agarose Bead
DEAERR-25 25 ml
EUR 102
DEAE Rapid Run Agarose Bead
DEAERR-300 300 ml
EUR 492
CM Rapid Run Agarose Bead
CMRR-25 25 ml
EUR 102
CM Rapid Run Agarose Bead
CMRR-300 300 ml
EUR 492
SP Rapid Run Agarose Bead
SPRR-25 25 ml
EUR 102
SP Rapid Run Agarose Bead
SPRR-300 300 ml
EUR 492
MS ELISA KIT|Human
EF006989 96 Tests
EUR 689
Anti-Ms Blnk Purified
11-411-C025 0.025 mg
EUR 122
Anti-Ms Blnk Purified
11-411-C100 0.1 mg
EUR 204
Anti-Ms CD45 Purified
11-553-C100 0.1 mg
EUR 113
Anti-Ms CD9 Purified
11-567-C100 0.1 mg
EUR 122
Anti-Ms Bim Purified
11-569-C025 0.025 mg
EUR 149
Anti-Ms Bim Purified
11-569-C100 0.1 mg
EUR 258
Anti-Ms CD3 Purified
11-578-C025 0.025 mg
EUR 76
Anti-Ms CD3 Purified
11-578-C100 0.1 mg
EUR 113
Anti-Ms CD8a Purified
11-579-C025 0.025 mg
EUR 76
Anti-Ms CD8a Purified
11-579-C100 0.1 mg
EUR 113
Anti-Ms CD4 Purified
11-580-C025 0.025 mg
EUR 76
Anti-Ms CD4 Purified
11-580-C100 0.1 mg
EUR 113
Anti-Ms CD79b Purified
11-584-C100 0.1 mg
EUR 149
Anti-Ms CD11b Purified
11-595-C100 0.1 mg
EUR 113
Anti-Ms CD19 Purified
11-596-C025 0.025 mg
EUR 76

Phosphoproteomic outcomes have been confirmed by Western blot and PBMCs’ confocal microscopy analyses. General, 38 PBMCs samples have been analyzed. A differential evaluation of PBMCs’ phosphoproteomes revealed a rise of lactotransferrin, actin-related protein 2 (ARPC2) and calgranulin-B in antibody-mediated rejection sufferers, in comparison with controls. Elevated expression of phosphorylated ARPC2 and its correlation to F-actin filaments have been confirmed in CAMR sufferers. Our outcomes are the primary proof of altered cytoskeleton group in circulating immune cells of CAMR sufferers.

 

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