Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum third-stage larvae (L3) are fish-borne nematodes that may trigger human anisakidosis. Though A. simplex is a identified supply of allergens, information concerning the allergic potential of P. decipiens and C. osculatum is proscribed. Subsequently, we carried out comparative proteomic profiling of A. simplex, P. decipiens, and C. osculatum L3 larvae utilizing liquid chromatography-tandem mass spectrometry. In whole, 645, 397, and 261 proteins had been detected in A. simplex, P. decipiens, and C. osculatum L3 larvae, respectively.
Western blot evaluation confirmed the cross-reactivity of anti-A. simplex immunoglobulin (Ig)G antibodies with protein extracts from P. decipiens and C. osculatum L3 larvae. The recognized proteins of the Anisakidae proteomes had been characterised by label-free quantification and practical evaluation, and proteins concerned in lots of important organic mechanisms, corresponding to parasite survival, had been recognized. In C. osculatum 4, the next allergens had been recognized: Ani s 2, Ani s 5, Ani s 13, and Asc l 3.
Moreover, 28 possible allergens had been predicted in A. simplex and P. decipiens, whereas in C. osculatum, 25 potential allergens had been recognized. Among the many putative allergens, warmth shock proteins had been most often detected, adopted by paramyosin, peptidyl-prolyl cis-trans isomerase, enolase, and tropomyosin. We offer a brand new proteomic information set that could possibly be helpful for the invention of biomarkers or drug goal candidates. Moreover, our findings confirmed that along with A. simplex, P. decipiens and C. osculatum also needs to be thought-about as potential sources of allergens that might result in IgE-mediated hypersensitivity.
The Position of RodA-Conserved Cysteine Residues within the Aspergillus fumigatus Conidial Floor Group
Immune inertness of Aspergillus fumigatus conidia is attributed to its floor rodlet-layer made up of RodAp, characterised by eight conserved cysteine residues forming 4 disulfide bonds. Earlier, we confirmed that the conserved cysteine residue level (ccrp) mutations end in conidia devoid of the rodlet layer. Right here, we prolonged our research evaluating the floor group and immunoreactivity of conidia carrying ccrp-mutations with the RODA deletion mutant (∆rodA). Western blot evaluation utilizing anti-RodAp antibodies indicated the absence of RodAp within the cytoplasm of ccrp–mutant conidia.
Immunolabeling revealed differential reactivity to conidial floor glucans, the ccrp-mutant conidia preferentially binding to α-(1,3)-glucan, ∆rodA conidia selectively sure to β-(1,3)-glucan; the parental pressure conidia confirmed adverse labeling. Nevertheless, permeability of ccrp-mutants and ∆rodA was much like the parental pressure conidia. Proteomic analyses of the conidial floor uncovered proteins of the ccrp-mutants confirmed extra similarities with the parental pressure, however had been considerably completely different from the ∆rodA. Ccrp-mutant conidia had been much less immunostimulatory in comparison with ∆rodA conidia.
Our information counsel that (i) the conserved cysteine residues are important for the trafficking of RodAp and the group of the rodlet layer on the conidial floor, and (ii) focused level mutation could possibly be another method to check the function of fungal cell-wall genes in host-fungal interplay. Aquaporin-2 (AQP2) is a key water channel protein which determines the water permeability of the amassing duct. A number of phosphorylation websites are current on the C-terminal of AQP2 together with S256 (serine at 256 residue), S261, S264 and S/T269, that are regulated by vasopressin (VP) to modulate AQP2 trafficking.
Because the dynamics of those phosphorylations have been studied largely in rodents, little is thought concerning the phosphorylation of human AQP2 which has distinctive T269 within the place of S269 of rodent AQP2. As a result of AQP2 is excreted in urinary exosomes, the phosphoprotein profile of human AQP2 might be simply examined by means of urinary exosomes with none intervention.
Steel Assisted Protein quantitation (MAPq): Multiplex evaluation of protein expression utilizing lanthanide-modified antibodies with detection by inductively coupled plasma mass spectrometry.
Understanding the advanced relationships between genomics, transcriptomics, and proteomics requires growth of extra delicate and speedy strategies of multiplexed protein evaluation. That is needed to know the connection between mobile responses to environmental stresses, illness development, and/or drug therapy; nevertheless, most strategies are restricted by low sensitivity, non-specificity, and minimal multiplexing capability. To extra totally discover the connection between a number of mobile pathways, we now have developed a novel antibody-based multiplex assay utilizing inductively coupled plasma mass spectrometry (ICP-MS), which we time period steel assisted protein quantitation (MAPq).
MAPq makes use of lanthanide-conjugated antibodies to concurrently quantify as much as 35 proteins with low pg/mL sensitivity. This methodology is very advantageous for low abundance proteins, a big limitation of many multiplex MS strategies. We noticed a restrict of detection of 0.5 pg/mL and restrict of quantitation of 5 pg/mL with nearly no background sign. We utilized this methodology to each cultured cells and mouse tissues to research modifications in low abundance nuclear and cytoplasmic proteins following drug or environmental stresses.
MAPq was discovered to be at the very least 10-times extra delicate than western blots and will detect quantitative modifications in protein expression not readily noticed utilizing standard approaches. The proteins had been confirmed by immunoblot analyses utilizing independently acquired urinary EV samples. AZGP1 specifically was discovered to be a CAMR-specific proteomic biomarker that was distinguishable from the rejection-free management group with matching kidney operate, period of transplantation, and age.
 Coomassie brilliant blue G-250 |
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| 20-abx082402 | Abbexa |
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Coomassie brilliant blue G-250 |
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| 20-abx082562 | Abbexa |
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Coomassie brilliant blue G-250 |
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| CB0038 | Bio Basic | 25g | EUR 75.23 |
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 Brilliant Blue FCF |
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| HY-D0915 | MedChemExpress | 500mg | EUR 108 |
 Coomassie Brilliant Blue G250 |
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| abx090654-5g | Abbexa | 5 g | EUR 189 |
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 Coomassie Brilliant Blue R250 |
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| abx090655-5g | Abbexa | 5 g | EUR 189 |
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) OPTIONAL BLUE FILTER (497NM) |
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| GDBL-BLUE | CORNING | 1/pk | EUR 1082 |
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Description: Lab Equipment; Axygen Branded EQ |
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 conversion screen uv/blue |
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| VLFC26-BLUE | Consort | ea | EUR 606 |
 Coomassie brilliant blue R-250 |
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| 20-abx082400 | Abbexa |
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Coomassie brilliant blue R-250 |
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| 20-abx082561 | Abbexa |
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 Coomassie Brilliant Blue R-250 |
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| CH026 | ABM | 50 g | EUR 175 |
) Brilliant Cresyl Blue (0.3%, Alcoholic) |
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| BCA3800 | ScyTek Laboratories | 1 Gal. | EUR 241 |
Brilliant Cresyl Blue (0.3%, Alcoholic) |
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| BCA500 | ScyTek Laboratories | 500 ml | EUR 90 |
Brilliant Cresyl Blue (0.3%, Alcoholic) |
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| BCA999 | ScyTek Laboratories | 1000 ml | EUR 116 |
) Brilliant Cresyl Blue (C.I. 51010) |
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| GT9275-10G | Glentham Life Sciences | 10 g | EUR 86 |
Brilliant Cresyl Blue (C.I. 51010) |
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| GT9275-25G | Glentham Life Sciences | 25 g | EUR 110 |
Coomassie brilliant blue R-250 |
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| CB0037 | Bio Basic | 5g | EUR 58.7 |
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 Coomassie Brilliant Blue Fast Staining Solution |
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| abx090652-200ml | Abbexa | 200 ml | EUR 203 |
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 Coomassie Brilliant Blue Gel Staining Kit |
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| abx090653-1Kit | Abbexa | 1 Kit | EUR 189 |
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) Coomassie Brilliant Blue R250 (C.I. 42660) |
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| GT3543-100G | Glentham Life Sciences | 100 g | EUR 126 |
Coomassie Brilliant Blue R250 (C.I. 42660) |
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| GT3543-25G | Glentham Life Sciences | 25 g | EUR 62 |
Coomassie Brilliant Blue R250 (C.I. 42660) |
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| GT3543-5G | Glentham Life Sciences | 5 g | EUR 46 |
) Remazol Brilliant Blue R (C.I. 61200) |
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| GT9632-25G | Glentham Life Sciences | 25 g | EUR 57 |
) Coomassie Brilliant Blue G250 (C.I. 42655) |
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| GT0353-100G | Glentham Life Sciences | 100 g | EUR 126 |
Coomassie Brilliant Blue G250 (C.I. 42655) |
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| GT0353-25G | Glentham Life Sciences | 25 g | EUR 62 |
Coomassie Brilliant Blue G250 (C.I. 42655) |
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| GT0353-5G | Glentham Life Sciences | 5 g | EUR 46 |
) Brilliant Cresyl Blue (1.5% in 0.85% Saline) |
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| BCS3800 | ScyTek Laboratories | 1 Gal. | EUR 241 |
Brilliant Cresyl Blue (1.5% in 0.85% Saline) |
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| BCS500 | ScyTek Laboratories | 500 ml | EUR 90 |
Brilliant Cresyl Blue (1.5% in 0.85% Saline) |
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| BCS999 | ScyTek Laboratories | 1000 ml | EUR 116 |
 Coomassie brilliant blue R-250 2 xSolution |
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| C462151 | Bio Basic | 250ml | EUR 106.55 |
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 Agarose for DNA electrophoresis |
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| 16-0027-500 | Medicago | 500 g | EUR 342 |
, for electrophoresis) Starch (Smithies), for electrophoresis |
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| GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 309 |
Starch (Smithies), for electrophoresis |
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| GE6857-500G | Glentham Life Sciences | 500 g | EUR 181 |
 Acrylamide, suitable for electrophoresis |
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| GK5651-100G | Glentham Life Sciences | 100 g | EUR 54 |
 10G Coomassie Blue G-250 |
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| NAT1362 | National Diagnostics | 10G | EUR 148 |
 Brilliant green |
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| BB0242 | Bio Basic | 25g | EUR 56.09 |
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 BRILLIANT GREEN AGAR |
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| B02-115-10kg | Alphabiosciences | 10 kg | EUR 1222 |
BRILLIANT GREEN AGAR |
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| B02-115-2Kg | Alphabiosciences | 2 Kg | EUR 305 |
BRILLIANT GREEN AGAR |
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| B02-115-500g | Alphabiosciences | 500 g | EUR 119 |
 CometAssay Electrophoresis System |
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| 4250-050-ES | Biotechne | 1 Kit | EUR 2950.8 |
 Electrophoresis System Set |
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| E1101-E | GeneOn | 1 pcs | Ask for price |
 electrophoresis power supply |
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| EV245 | Consort | ea | EUR 479 |
 ES 6 Tooth Gel Comb For ES Electrophoresis |
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| LE1011-A4 | GenDepot | Ea | EUR 124 |
 Blue Base for Dissecting Board |
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| M625 | ScyTek Laboratories | 1 ea. | EUR 200 |
 BRILLIANT GREEN BILE AGAR |
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| B02-122-10kg | Alphabiosciences | 10 kg | EUR 1267 |
BRILLIANT GREEN BILE AGAR |
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| B02-122-2Kg | Alphabiosciences | 2 Kg | EUR 315 |
BRILLIANT GREEN BILE AGAR |
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| B02-122-500g | Alphabiosciences | 500 g | EUR 122 |
) Brilliant Green (C.I. 42040) |
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| GT6123-100G | Glentham Life Sciences | 100 g | EUR 78 |
Brilliant Green (C.I. 42040) |
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| GT6123-25G | Glentham Life Sciences | 25 g | EUR 46 |
) Brilliant Crocein (C.I. 27290) |
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| GT8393-100G | Glentham Life Sciences | 100 g | EUR 86 |
Brilliant Crocein (C.I. 27290) |
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| GT8393-250G | Glentham Life Sciences | 250 g | EUR 150 |
Brilliant Crocein (C.I. 27290) |
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| GT8393-50G | Glentham Life Sciences | 50 g | EUR 62 |
 CometAssay Electrophoresis Starter Kit |
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| 4250-050-ESK | Biotechne | 1 Kit | EUR 3124.4 |
 myGel Mini Electrophoresis System |
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| BM0286 | GenDepot | System | EUR 618 |
 ES Horizontal Electrophoresis Set |
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| LE1011 | GenDepot | Set | EUR 482 |
 6X6 well Electrophoresis Apparatus |
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| EDK626 | Bio Basic | 1 UNIT | EUR 625.5 |
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 ES 8/10 Double Gel Comb For ES Electrophoresis |
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| LE1011-A3 | GenDepot | Ea | EUR 124 |
 ECOS Blue (XL1-Blue |
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| FYE107-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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| FYE107-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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| FYE108-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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| FYE108-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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| FYE109-10VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
ECOS Blue (XL1-Blue |
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| FYE109-80VL | Yeastern Biotech | 100 µl x 10 vials | Ask for price |
 BRILLIANT GREEN AGAR WITH SULFAPYRIDINE |
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| B02-117-10kg | Alphabiosciences | 10 kg | Ask for price |
BRILLIANT GREEN AGAR WITH SULFAPYRIDINE |
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| B02-117-2kg | Alphabiosciences | 2kg | Ask for price |
BRILLIANT GREEN AGAR WITH SULFAPYRIDINE |
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| B02-117-500g | Alphabiosciences | 500 g | Ask for price |
 BRILLIANT GREEN BILE BROTH 2% |
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| B02-118-10kg | Alphabiosciences | 10 kg | EUR 1022 |
BRILLIANT GREEN BILE BROTH 2% |
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| B02-118-2Kg | Alphabiosciences | 2 Kg | EUR 261 |
BRILLIANT GREEN BILE BROTH 2% |
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| B02-118-500g | Alphabiosciences | 500 g | EUR 107 |
) Brilliant Black BN (C.I. 28440) |
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| GT6953-100G | Glentham Life Sciences | 100 g | EUR 140 |
Brilliant Black BN (C.I. 28440) |
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| GT6953-10G | Glentham Life Sciences | 10 g | EUR 54 |
Brilliant Black BN (C.I. 28440) |
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| GT6953-25G | Glentham Life Sciences | 25 g | EUR 78 |
 Cibacron Brilliant Red 3B-A |
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| GT9393-25G | Glentham Life Sciences | 25 g | EUR 181 |
Cibacron Brilliant Red 3B-A |
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| GT9393-5G | Glentham Life Sciences | 5 g | EUR 78 |
 electrophoresis ps 400v, 500ma, 50w |
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| EV1450 | Consort | ea | EUR 479 |
electrophoresis ps 400v, 500ma, 50w |
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| EV1450-UK | Consort | ea | EUR 479 |
electrophoresis ps 400v, 500ma, 50w |
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| EV1450-US | Consort | ea | EUR 479 |
 electrophoresis ps 1500v, 300ma, 150w |
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| EV2230 | Consort | ea | EUR 749 |
electrophoresis ps 1500v, 300ma, 150w |
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| EV2230-UK | Consort | ea | EUR 749 |
electrophoresis ps 1500v, 300ma, 150w |
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| EV2230-US | Consort | ea | EUR 749 |
 electrophoresis ps 300v, 1000ma, 150w |
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| EV2310 | Consort | ea | EUR 572 |
electrophoresis ps 300v, 1000ma, 150w |
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| EV2310-UK | Consort | ea | EUR 572 |
electrophoresis ps 300v, 1000ma, 150w |
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| EV2310-US | Consort | ea | EUR 572 |
 electrophoresis ps 3000v, 150ma, 150w |
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| EV2320 | Consort | ea | EUR 749 |
electrophoresis ps 3000v, 150ma, 150w |
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| EV2320-UK | Consort | ea | EUR 749 |
electrophoresis ps 3000v, 150ma, 150w |
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| EV2320-US | Consort | ea | EUR 749 |
 electrophoresis power supply uk plug |
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| EV245-UK | Consort | ea | EUR 479 |
 electrophoresis power supply 120 vac |
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| EV245-US | Consort | ea | EUR 479 |
 electrophoresis ps 600v, 500ma, 150w |
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| EV2650 | Consort | ea | EUR 572 |
electrophoresis ps 600v, 500ma, 150w |
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| EV2650-UK | Consort | ea | EUR 572 |
electrophoresis ps 600v, 500ma, 150w |
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| EV2650-US | Consort | ea | EUR 572 |
 electrophoresis ps 300v, 2000ma, 300w |
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| EV3020 | Consort | ea | EUR 800 |
electrophoresis ps 300v, 2000ma, 300w |
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| EV3020-UK | Consort | ea | EUR 800 |
electrophoresis ps 300v, 2000ma, 300w |
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| EV3020-US | Consort | ea | EUR 800 |
 electrophoresis ps 1200v, 500ma, 300w |
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| EV3150 | Consort | ea | EUR 800 |
electrophoresis ps 1200v, 500ma, 300w |
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| EV3150-UK | Consort | ea | EUR 800 |
electrophoresis ps 1200v, 500ma, 300w |
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| EV3150-US | Consort | ea | EUR 800 |
 electrophoresis ps 3000v, 300ma, 300w |
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| EV3330 | Consort | ea | EUR 1214 |
electrophoresis ps 3000v, 300ma, 300w |
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| EV3330-UK | Consort | ea | EUR 1214 |
electrophoresis ps 3000v, 300ma, 300w |
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| EV3330-US | Consort | ea | EUR 1214 |
 electrophoresis ps 600v, 1000ma, 300w |
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| EV3610 | Consort | ea | EUR 800 |
electrophoresis ps 600v, 1000ma, 300w |
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| EV3610-UK | Consort | ea | EUR 800 |
electrophoresis ps 600v, 1000ma, 300w |
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| EV3610-US | Consort | ea | EUR 800 |
 electrophoresis ps 6000v, 150ma, 300w |
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| EV3620 | Consort | ea | EUR 1214 |
electrophoresis ps 6000v, 150ma, 300w |
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| EV3620-UK | Consort | ea | EUR 1214 |
electrophoresis ps 6000v, 150ma, 300w |
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| EV3620-US | Consort | ea | EUR 1214 |
 Antibody (CF-Blue)) Adhesion G Protein-Coupled Receptor E1 (ADGRE1) Antibody (CF-Blue) |
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| 20-abx200932 | Abbexa |
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 Phthalocyanine blue |
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| 20-abx181340 | Abbexa |
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 Evans Blue |
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| abx186080-125g | Abbexa | 125g | EUR 453 |
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 Bromophenol blue |
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| abx183916-500ml | Abbexa | 500 ml | EUR 230 |
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Bromophenol blue |
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| abx183916-5l | Abbexa | 5l | EUR 592 |
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Evans Blue |
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| 20-abx082082 | Abbexa |
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 Bromphenol Blue |
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| 20-abx082184 | Abbexa |
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Evans Blue |
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| 20-abx082237 | Abbexa |
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Bromphenol Blue |
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| 20-abx082376 | Abbexa |
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 Toluidine Blue |
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| abx082425-5g | Abbexa | 5 g | EUR 189 |
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 Trypan Blue |
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| 20-abx082476 | Abbexa |
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 Bromophenol Blue |
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| abx082607-10g | Abbexa | 10 g | EUR 203 |
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Bromophenol Blue |
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| CH019 | ABM | 25 g | EUR 132 |
Bromophenol Blue |
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| CH020 | ABM | 50 g | EUR 166 |
Bromophenol Blue |
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| CH021 | ABM | 100 g | EUR 227 |
Bromophenol Blue |
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| B7774-5000 | ApexBio | 5 g | EUR 87 |
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Description: IC50: N/ABromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is widely used in gel loading buffers. Bromophenol blue is experimentally used as acolor marker, apH indicator, and a dye. |
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 Methylene Blue |
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| HY-14536 | MedChemExpress | 100mg | EUR 119 |
 Thiazolyl Blue |
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| HY-15924 | MedChemExpress | 10mM/1mL | EUR 113 |
Evans Blue |
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| HY-B1102 | MedChemExpress | 100mg | EUR 119 |
 Bromothymol Blue |
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| HY-D0012 | MedChemExpress | 1g | EUR 119 |
 Diphenyl Blue |
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| HY-D0970 | MedChemExpress | 10mM/1mL | EUR 113 |
 Calcein Blue |
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| HY-101887 | MedChemExpress | 25mg | EUR 119 |
 Bromochlorophenol Blue |
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| GT2674-100MG | Glentham Life Sciences | 100 mg | EUR 78 |
Bromochlorophenol Blue |
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| GT2674-1G | Glentham Life Sciences | 1 g | EUR 174 |
Bromophenol Blue |
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| GT2874-100G | Glentham Life Sciences | 100 g | EUR 126 |
Bromophenol Blue |
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| GT2874-10G | Glentham Life Sciences | 10 g | EUR 52 |
Bromophenol Blue |
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| GT2874-25G | Glentham Life Sciences | 25 g | EUR 66 |
Bromophenol Blue |
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| GT2874-50G | Glentham Life Sciences | 50 g | EUR 86 |
Bromophenol Blue |
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| GT2874-5G | Glentham Life Sciences | 5 g | EUR 45 |
 Hydroxynaphthol blue |
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| GT3182-100G | Glentham Life Sciences | 100 g | EUR 237 |
Hydroxynaphthol blue |
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| GT3182-10G | Glentham Life Sciences | 10 g | EUR 78 |
Hydroxynaphthol blue |
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| GT3182-25G | Glentham Life Sciences | 25 g | EUR 110 |
Hydroxynaphthol blue |
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| GT3182-50G | Glentham Life Sciences | 50 g | EUR 166 |
Bromothymol Blue |
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| GT6837-10G | Glentham Life Sciences | 10 g | EUR 47 |
Bromothymol Blue |
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| GT6837-25G | Glentham Life Sciences | 25 g | EUR 61 |
Bromothymol Blue |
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| GT6837-50G | Glentham Life Sciences | 50 g | EUR 86 |
Bromothymol Blue |
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| GT6837-5G | Glentham Life Sciences | 5 g | EUR 42 |
 Xylenol Blue |
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| GT7988-1G | Glentham Life Sciences | 1 g | EUR 46 |
Xylenol Blue |
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| GT7988-25G | Glentham Life Sciences | 25 g | EUR 150 |
Xylenol Blue |
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| GT7988-5G | Glentham Life Sciences | 5 g | EUR 78 |
 Bromoxylenol Blue |
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| GT8506-10G | Glentham Life Sciences | 10 g | EUR 142 |
Bromoxylenol Blue |
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| GT8506-1G | Glentham Life Sciences | 1 g | EUR 46 |
Bromoxylenol Blue |
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| GT8506-5G | Glentham Life Sciences | 5 g | EUR 94 |
 Thymol Blue |
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| GT9755-100G | Glentham Life Sciences | 100 g | EUR 150 |
Thymol Blue |
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| GT9755-10G | Glentham Life Sciences | 10 g | EUR 50 |
Thymol Blue |
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| GT9755-25G | Glentham Life Sciences | 25 g | EUR 70 |
Thymol Blue |
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| GT9755-50G | Glentham Life Sciences | 50 g | EUR 94 |
Thymol Blue |
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| GT9755-5G | Glentham Life Sciences | 5 g | EUR 42 |
Bromophenol Blue |
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| G114 | ABM | 5.0 g | EUR 102 |
 Meldola's blue |
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| GT0259-10G | Glentham Life Sciences | 10 g | EUR 86 |
Meldola's blue |
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| GT0259-25G | Glentham Life Sciences | 25 g | EUR 134 |
Meldola's blue |
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| GT0259-50G | Glentham Life Sciences | 50 g | EUR 213 |
Bromophenol blue |
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| BB2230 | Bio Basic | 25g | EUR 69.14 |
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 Trypan blue |
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| TT1140 | Bio Basic | 10g | EUR 65.66 |
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 For-Met-Ala-OH |
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| G-1855.0250 | Bachem | 250.0mg | EUR 187 |
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Description: Sum Formula: C9H16N2O4S; CAS# [15183-28-5] |
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For-Met-Ala-OH |
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| G-1855.1000 | Bachem | 1.0g | EUR 502 |
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Description: Sum Formula: C9H16N2O4S; CAS# [15183-28-5] |
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We recognized and validated six proteomic biomarkers for CAMR and clarified one CAMR-specific proteomic biomarker in KTRs. Additional scientific trials are wanted earlier than these rejection-specific biomarkers might be utilized for the early prediction, analysis, and monitoring of the scientific response of KTRs to the therapy of CAMR.