Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum

Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum third-stage larvae (L3) are fish-borne nematodes that may trigger human anisakidosis. Though A. simplex is a identified supply of allergens, information concerning the allergic potential of P. decipiens and C. osculatum is proscribed. Subsequently, we carried out comparative proteomic profiling of A. simplex, P. decipiens, and C. osculatum L3 larvae utilizing liquid chromatography-tandem mass spectrometry. In whole, 645, 397, and 261 proteins had been detected in A. simplex, P. decipiens, and C. osculatum L3 larvae, respectively.

Western blot evaluation confirmed the cross-reactivity of anti-A. simplex immunoglobulin (Ig)G antibodies with protein extracts from P. decipiens and C. osculatum L3 larvae. The recognized proteins of the Anisakidae proteomes had been characterised by label-free quantification and practical evaluation, and proteins concerned in lots of important organic mechanisms, corresponding to parasite survival, had been recognized. In C. osculatum 4, the next allergens had been recognized: Ani s 2, Ani s 5, Ani s 13, and Asc l 3.

Moreover, 28 possible allergens had been predicted in A. simplex and P. decipiens, whereas in C. osculatum, 25 potential allergens had been recognized. Among the many putative allergens, warmth shock proteins had been most often detected, adopted by paramyosin, peptidyl-prolyl cis-trans isomerase, enolase, and tropomyosin. We offer a brand new proteomic information set that could possibly be helpful for the invention of biomarkers or drug goal candidates. Moreover, our findings confirmed that along with A. simplex, P. decipiens and C. osculatum also needs to be thought-about as potential sources of allergens that might result in IgE-mediated hypersensitivity.

The Position of RodA-Conserved Cysteine Residues within the Aspergillus fumigatus Conidial Floor Group

Immune inertness of Aspergillus fumigatus conidia is attributed to its floor rodlet-layer made up of RodAp, characterised by eight conserved cysteine residues forming 4 disulfide bonds. Earlier, we confirmed that the conserved cysteine residue level (ccrp) mutations end in conidia devoid of the rodlet layer. Right here, we prolonged our research evaluating the floor group and immunoreactivity of conidia carrying ccrp-mutations with the RODA deletion mutant (∆rodA). Western blot evaluation utilizing anti-RodAp antibodies indicated the absence of RodAp within the cytoplasm of ccrpmutant conidia.

Immunolabeling revealed differential reactivity to conidial floor glucans, the ccrp-mutant conidia preferentially binding to α-(1,3)-glucan, ∆rodA conidia selectively sure to β-(1,3)-glucan; the parental pressure conidia confirmed adverse labeling. Nevertheless, permeability of ccrp-mutants and ∆rodA was much like the parental pressure conidiaProteomic analyses of the conidial floor uncovered proteins of the ccrp-mutants confirmed extra similarities with the parental pressure, however had been considerably completely different from the ∆rodA. Ccrp-mutant conidia had been much less immunostimulatory in comparison with ∆rodA conidia.

Our information counsel that (i) the conserved cysteine residues are important for the trafficking of RodAp and the group of the rodlet layer on the conidial floor, and (ii) focused level mutation could possibly be another method to check the function of fungal cell-wall genes in host-fungal interplay. Aquaporin-2 (AQP2) is a key water channel protein which determines the water permeability of the amassing duct. A number of phosphorylation websites are current on the C-terminal of AQP2 together with S256 (serine at 256 residue), S261, S264 and S/T269, that are regulated by vasopressin (VP) to modulate AQP2 trafficking.

Because the dynamics of those phosphorylations have been studied largely in rodents, little is thought concerning the phosphorylation of human AQP2 which has distinctive T269 within the place of S269 of rodent AQP2. As a result of AQP2 is excreted in urinary exosomes, the phosphoprotein profile of human AQP2 might be simply examined by means of urinary exosomes with none intervention.

Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum

Steel Assisted Protein quantitation (MAPq): Multiplex evaluation of protein expression utilizing lanthanide-modified antibodies with detection by inductively coupled plasma mass spectrometry.

Understanding the advanced relationships between genomics, transcriptomics, and proteomics requires growth of extra delicate and speedy strategies of multiplexed protein evaluation. That is needed to know the connection between mobile responses to environmental stresses, illness development, and/or drug therapy; nevertheless, most strategies are restricted by low sensitivity, non-specificity, and minimal multiplexing capability. To extra totally discover the connection between a number of mobile pathways, we now have developed a novel antibody-based multiplex assay utilizing inductively coupled plasma mass spectrometry (ICP-MS), which we time period steel assisted protein quantitation (MAPq).

MAPq makes use of lanthanide-conjugated antibodies to concurrently quantify as much as 35 proteins with low pg/mL sensitivity. This methodology is very advantageous for low abundance proteins, a big limitation of many multiplex MS strategies. We noticed a restrict of detection of 0.5 pg/mL and restrict of quantitation of 5 pg/mL with nearly no background sign. We utilized this methodology to each cultured cells and mouse tissues to research modifications in low abundance nuclear and cytoplasmic proteins following drug or environmental stresses.

MAPq was discovered to be at the very least 10-times extra delicate than western blots and will detect quantitative modifications in protein expression not readily noticed utilizing standard approaches. The proteins had been confirmed by immunoblot analyses utilizing independently acquired urinary EV samples. AZGP1 specifically was discovered to be a CAMR-specific proteomic biomarker that was distinguishable from the rejection-free management group with matching kidney operate, period of transplantation, and age.

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 74.66

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 139.51

electrophoresis power supply

EV245 ea
EUR 574.8

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

miniONE Electrophoresis System

ELE3400 EACH
EUR 691.2

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

myGel Mini Electrophoresis System

E1101 1 each
EUR 623.9

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
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Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
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Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
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Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
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electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
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CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
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Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

Brilliant Blue G

C5579-100000 100 g
EUR 162
Description: Coomassie Brilliant Blue G-250 is used for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method, with high band visibility.

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
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ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
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LE1002 Set
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LE1006 Set
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Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
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10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
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CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
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myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
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myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
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CometAssay Electrophoresis Starter Kit plus SZ Power Supply

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ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

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Coomasie Brilliant Blue G-250

A2329-25G 25G
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Description: Ultra Pure

Coomasie Brilliant Blue G-250

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Description: Ultra Pure

Coomassie brilliant blue G-250

20-abx082402
  • EUR 276.00
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Coomassie brilliant blue G-250

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  • EUR 260.40
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Coomassie brilliant blue G-250

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Coomassie Brilliant Blue G-250

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Coomassie Brilliant Blue G-250

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Coomassie Brilliant Blue G-250

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Coomassie Brilliant Blue G-250

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Brilliant Blue FCF

HY-D0915 500mg
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myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

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High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
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myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
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Coomassie Brilliant Blue 0.125%

40120761-1 1 L
EUR 77.66

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
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Coomassie Brilliant Blue G250

abx090654-5g 5 g
EUR 226.8

Coomassie Brilliant Blue R250

abx090655-5g 5 g
EUR 226.8

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

Remazol Brilliant Blue R (C.I. 61200)

GT9632-25G 25 g
EUR 68.4

Remazol Brilliant Blue R (C.I. 61200)

GT9632-25 25
EUR 26.9

Remazol Brilliant Blue R (C.I. 61200)

GT9632-5 5
EUR 15.3

Coomassie Brilliant Blue Solution

GR103030 100 mL
EUR 83

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GT9275-10G 10 g
EUR 103.2

Brilliant Cresyl Blue (C.I. 51010)

GT9275-25G 25 g
EUR 132

Brilliant Cresyl Blue (C.I. 51010)

GT9275-10 10
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Brilliant Cresyl Blue (C.I. 51010)

GT9275-25 25
EUR 79.1

Coomasie Brilliant Blue R-250

A2330-25G 25G
EUR 35.2
Description: Ultra Pure

Coomasie Brilliant Blue R-250

A2330-5G 5G
EUR 9.9
Description: Ultra Pure

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

Coomassie Brilliant Blue R-250

CH024 10 g
EUR 145.2

Coomassie Brilliant Blue R-250

CH025 25 g
EUR 174

Coomassie Brilliant Blue R-250

CH026 50 g
EUR 210

Coomassie brilliant blue R-250

20-abx082400
  • EUR 276.00
  • EUR 226.80
  • 25 g
  • 5 g

Coomassie brilliant blue R-250

20-abx082561
  • EUR 260.40
  • EUR 210.00
  • 25 g
  • 5 g

Coomassie brilliant blue R-250

CB0037 5g
EUR 70.44

Coomassie Brilliant Blue R-250

40340012-1 10 g
EUR 20.06

Coomassie Brilliant Blue R-250

40340012-2 25 g
EUR 38.77

Coomassie Brilliant Blue R-250

40340012-3 50 g
EUR 72.08

Coomassie Brilliant Blue R-250

40340012-4 100 g
EUR 109.36

Coomassie Brilliant Blue G250 (C.I. 42655)

GT0353-100G 100 g
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Coomassie Brilliant Blue G250 (C.I. 42655)

GT0353-25G 25 g
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Coomassie Brilliant Blue G250 (C.I. 42655)

GT0353-5G 5 g
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Coomassie Brilliant Blue R250 (C.I. 42660)

GT3543-100G 100 g
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Coomassie Brilliant Blue R250 (C.I. 42660)

GT3543-25G 25 g
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Coomassie Brilliant Blue R250 (C.I. 42660)

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Coomassie Brilliant Blue G250 (C.I. 42655)

GT0353-100 100
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Coomassie Brilliant Blue G250 (C.I. 42655)

GT0353-25 25
EUR 31.7

Coomassie Brilliant Blue G250 (C.I. 42655)

GT0353-5 5
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Coomassie Brilliant Blue R250 (C.I. 42660)

GT3543-100 100
EUR 95

Coomassie Brilliant Blue R250 (C.I. 42660)

GT3543-25 25
EUR 31.7

Coomassie Brilliant Blue R250 (C.I. 42660)

GT3543-5 5
EUR 15.9

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA3800 1 Gal.
EUR 289.2

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA500 500 ml
EUR 108

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA999 1000 ml
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Brilliant Cresyl Blue 0.5% Alcoholic

RRSP13-B 100ml
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Brilliant Cresyl Blue 0.5% Alcoholic

RRSP13-C 250ml
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Brilliant Cresyl Blue 0.5% Alcoholic

RRSP13-D 500ml
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Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 226.8

Coomassie Brilliant Blue Fast Staining Solution

abx090652-200ml 200 ml
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Coomassie brilliant blue R-250 2 xSolution

C462151 250ml
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Brilliant Cresyl Blue (1.5% in 0.85% Saline)

BCS3800 1 Gal.
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Brilliant Cresyl Blue (1.5% in 0.85% Saline)

BCS500 500 ml
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Brilliant Cresyl Blue (1.5% in 0.85% Saline)

BCS999 1000 ml
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Brilliant Cresyl Blue in Citrate Saline

RRSP14-B 100ml
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Brilliant Cresyl Blue in Citrate Saline

RRSP14-C 250ml
EUR 19.31

Brilliant Cresyl Blue in Citrate Saline

RRSP14-D 500ml
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Brilliant green

BB0242 25g
EUR 67.31

Brilliant Green

21770007-1 100 mL
EUR 69.54

Brilliant Green

21770007-2 500 mL
EUR 107.06

Brilliant Green

21770007-3 1 L
EUR 172.95

Brilliant Green

21770007-4 4 L
EUR 317.99

Brilliant Green

A4154-100G 100G
EUR 52.8
Description: Biotechnology

Brilliant Green

A4154-10G 10G
EUR 15.4
Description: Biotechnology

Brilliant Green

A4154-25G 25G
EUR 22
Description: Biotechnology

We recognized and validated six proteomic biomarkers for CAMR and clarified one CAMR-specific proteomic biomarker in KTRs. Additional scientific trials are wanted earlier than these rejection-specific biomarkers might be utilized for the early prediction, analysis, and monitoring of the scientific response of KTRs to the therapy of CAMR.

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