Properties
Quality level: 100
Biological source: human
Test
- > 97% (SDS-PAGE and HPLC)
- > 98% (SDS-PAGE and HPLC)
mole weight: Mw 15.5 kDa
mfr. Not.: Chemicon®
Application(s)
cell analysis: adequate (cell culture)
impurities
- <0.1 ng / μg endotoxin (from IL-2)
- 1 EU / μg
Input
hematopoietic stem cell sample type
NCBI access no.: NM_000586.3
UniProt access no.: P60568
Related categories
Growth factors and cytokines
General description
Interleukin-2 (IL-2) is a potent lymphoid cell growth factor that exerts its biological activity mainly on T cells. Human IL-2 is a 15-16 kDa protein that contains 159 amino acid residues. This preparation contains monomeric and dimeric forms of IL-2.
Request
For most in vitro applications, IL-2 exerts its biological activity in the concentration range of 0.1 to 1.0 ng / mL. The cells that respond are (partial list): T cells, NK cells, LAK cells.
Storage and stability
Lyophilized IL-2, although stable at room temperature, is best stored at -20 ° C. After rapid centrifugation, reconstitute in 100 mM acetic acid to a concentration of 0.1-1 mg/ml. This solution can be diluted in other buffered solutions or stored. Reconstituted IL-2 should be stored in undiluted aliquots at -20 ° C for up to six months. Avoid repeated freeze/thaw cycles.
Legal information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany